MAKING COLLAGEN-CHITOSAN SCAFFOLD AS A DELIVERY SYSTEM FOR TOPPASPARA LEAF EXTRACT (Mikania micrantha Kunth)
DOI:
10.013201/zrnt0b39Downloads
Abstract
Wounds are damage to the skin accompanied by loss of continuity of epithelial tissue with or without damage to other tissues such as muscles, bones and nerves. Scaffolds with porous structures are important in manipulating cell function and supporting the formation of new organs. Chitosan has been studied to be able to stimulate cell proliferation, increase collagenization, and accelerate cell regeneration (reepithelialization) in injured skin. Collagen is known to be the most promising material and has been found in various applications in tissue engineering because it has good biocompatibility, biodegradability and low antigenic properties. In this study, the materials used to make the scaffold were chitosan and collagen with the addition of glycerol and toppaspara leaf extract at a concentration of 1% 2% 3%. And the results are in quality testing of scaffold characteristics including SEM test, tensile test, cytotoxicity test. Next, we tested the healing of artificial wounds on mouse skin. The results of the research show that toppaspara leaf extract can be formulated into a collagen-chitosan scaffold dosage form with the addition of glycerol, has good physical quality characteristics for SEM tests, tensile tests, cytotoxicity tests, degradation tests, and thickness tests. And the ability to heal artificial wounds in mice was that on the 19th day there was no significant difference in the percentage of wound healing between the groups given Binasol Ointment, with 2% EEDTp and 3% EEDTp, and between those given 2% EEDTp and 3% EEDTp.
Keywords:
Chitosan collagen wound scaffold toppaspara leaf extractReferences
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